Comparisons of rotavirus polypeptides by limited proteolysis: close similarity of certain polypeptides of different strains
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چکیده
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Controlled Proteolysis of Nascent Polypeptides in Rat Liver Cell Fractions
Rough microsomes were incubated in an in vitro amino acid-incorporating system for labeling the nascent polypeptide chains on the membrane-bound ribosomes. Sucrose density gradient analysis showed that ribosomes did not detach from the membranes during incorporation in vitro. Trypsin and chymotrypsin treatment of microsomes at 0 degrees led to the detachment of ribosomes from the membranes; fur...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1981
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.40.3.720-728.1981